PDF chapter test TRY NOW
With a neat labelled diagram explain the techniques involved in gene cloning.
Answer variants:
Stanley Cohen
Inserting the DNA fragment into a suitable vector called plasmid to make rDNA
Selection and multiplication of recombinant host cell to obtain a clone
Expression of the cloned gene in a host cell
restriction enzymes
Clone
Salmonella typhimurium
Incorporation of rDNA into the bacterial host cell
Gene cloning
Herbert Boyer
Isolating the desired DNA fragment using restriction enzymes
a. is a carbon copy of an individual. It is a process of creating a genetically identical copy of an organism.
b. involves inserting a gene or a DNA fragment into a bacterial cell, which then multiplies (copies) the DNA when the cell divides. The basic steps involved in gene cloning is outlined below:
Step \(1\):
Step \(2\):
Step \(3\):
Step \(4\):
Step \(5\):
c. The first recombinant DNA was constructed by and in the year \(1972\).
d. A piece of DNA from a plasmid carrying antibiotic resistance gene in the bacterium was cut with the help of .
Register for free to see more content